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3-O-Acetyloleanolic Acid Induces Apoptosis in Human Colon Carcinoma Hct-116 Cells

Publication Type:Journal Article
Year of Publication:2012
Authors:Yoo, KHyun, Park, J-H, Cui, EJi, Kim, KIl, Kim, JYun, Kim, J, Hong, SGil, Baek, NIn, Chung, ISik
Journal:Phytotherapy Research
Volume:26
Issue:10
Date Published:2012
ISBN Number:1099-1573
Keywords:3-O-acetyloleanolic acid, apoptosis, colon carcinoma HCT-116 cells, death receptor DR5, Vigna
Abstract:

The cytotoxic effect of 3-O-acetyloleanolic acid, an oleanolic acid derivative isolated from the seeds of Vigna sinensis K., was investigated in human colon carcinoma HCT-116 cells. 3-O-acetyloleanolic acid dose-dependently inhibited the viability of HCT-116 cells. Apoptosis was characterized by detection of cell surface annexin V and sub-G1 apoptotic cell populations. The number of immunostained cells with annexin V-FITC was increased after treatment with 3-O-acetyloleanolic acid. The sub-G1 cell population was also increased. Expression of TRAIL-mediated apoptosis signaling-related death receptor DR5 was increased in 3-O-acetyloleanolic acid-treated HCT-116 cells. Activation of caspase-8 and caspase-3, critical mediators of extrinsic apoptosis signaling, was also increased by 3-O-acetyloleanolic acid. The results indicate that 3-O-acetyloleanolic acid induces apoptosis in HCT-116 cells mediated by an extrinsic apoptosis signaling cascade via up-regulation of DR5. Copyright © 2012 John Wiley & Sons, Ltd.

URL:http://dx.doi.org/10.1002/ptr.4616
Short Title:Phytotherapy Research
Taxonomic name: 
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