IBIS-Flora

Angiosperm Flora of India

Cryptogein, a fungal elicitor, remodels the phenylpropanoid metabolism of tobacco cell suspension cultures in a calcium-dependent manner

Publication Type:Journal Article
Year of Publication:2011
Authors:AMELOT, NICOLAS, CARROUCHE, AUDREY, DANOUN, SAÏDA, BOURQUE, STÉPHANE, HAIECH, JACQUES, Pugin, A, Ranjeva, R, Grima-Pettenati, J, Mazars, C, Brière, C
Journal:Plant, Cell & Environment
Volume:34
Issue:1
Date Published:2011
ISBN Number:1365-3040
Keywords:calcium signalling, Modelling, Nicotiana, Nicotiana tabacum, Nicotiana tabacum (tobacco BY-2), transcription regulation
Abstract:

Plant cells use calcium-based signalling pathways to transduce biotic and/or abiotic stimuli into adaptive responses. However, little is known about the coupling between calcium signalling, transcriptional regulation and the downstream biochemical processes. To understand these relationships better, we challenged tobacco BY-2 cells with cryptogein and evaluated how calcium transients (monitored through the calcium sensor aequorin) impact (1) transcript levels of phenylpropanoid genes (assessed by RT-qPCR); and (2) derived-phenolic compounds (analysed by mass spectrometry). Most genes of the phenylpropanoid pathway were up-regulated by cryptogein and cell wall-bound phenolic compounds accumulated (mainly 5-hydroxyferulic acid). The accumulation of both transcripts and phenolics was calcium-dependent. The transcriptional regulation of phenylpropanoid genes was correlated in a non-linear manner with stimulus intensity and with components of the cryptogein-induced calcium signature. In addition, calmodulin inhibitors increased the sensitivity of cells to low concentrations of cryptogein. These results led us to propose a model of coupling between the cryptogein signal, calcium signalling and the transcriptional response, exerting control of transcription through the coordinated action of two decoding modules exerting opposite effects.

URL:http://dx.doi.org/10.1111/j.1365-3040.2010.02233.x
Short Title:Plant, Cell & Environment
Fri, 2014-01-24 22:08 -- admin
https://secure.gravatar.com/avatar/5ade1b012674ce3dd941e2ea5dd15cc1.jpg?d=https%3A//flora.indianbiodiversity.org/sites/all/modules/patches/contrib/gravatar/avatar.png&s=100&r=G
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith