Publication Type: | Journal Article |
Year of Publication: | 2011 |
Authors: | Augustine, RC, Pattavina, KA, Tüzel, E, Vidali, L, Bezanilla, M |
Journal: | The Plant Cell |
Volume: | 23 |
Issue: | 10 |
Date Published: | 2011 |
ISBN Number: | 10404651 |
Abstract: | The remodeling of actin networks is required for a variety of cellular processes in eukaryotes. In plants, several actin binding proteins have been implicated in remodeling cortical actin filaments (F-actin). However, the extent to which these proteins support F-actin dynamics in planta has not been tested. Using reverse genetics, complementation analyses, and cell biological approaches, we assessed the in vivo function of two actin turnover proteins: actin interacting proteini (AIP1) and actin depolymerizing factor (ADF). We report that AIP1 is a single-copy gene in the moss Physcomitrella patens. AIP1 knockout plants are viable but have reduced expansion of tip-growing cells. AIP1 is diffusely cytosolic and functions in a common genetic pathway with ADF to promote tip growth. Specifically, ADF can partially compensate for loss of AIP1, and AIP1 requires ADF for function. Consistent with a role in actin remodeling, AIP1 knockout lines accumulate F-actin bundles, have fewer dynamic ends, and have reduced severing frequency. Importantly, we demonstrate that AIP1 promotes and ADF is essential for cortical F-actin dynamics. |
URL: | http://www.jstor.org/stable/41433871 |
Short Title: | The Plant Cell |