Angiosperm Flora of India

The APC/C subunit 10 plays an essential role in cell proliferation during leaf development

Publication Type:Journal Article
Year of Publication:2011
Authors:Eloy, NB, Lima, Mde Freitas, Van Damme, D, Vanhaeren, H, Gonzalez, N, De Milde, L, Hemerly, AS, Beemster, GTS, Inzé, D, Ferreira, PCG
Journal:The Plant Journal
Date Published:2011
ISBN Number:1365-313X
Keywords:APC/C, cell cycle, gametogenesis, leaf development, organ growth

The largest E3 ubiquitin-ligase complex, known as anaphase-promoting complex/cyclosome (APC/C), regulates the proteolysis of cell cycle regulators such as CYCLIN B and SECURIN that are essential for sister-chromatid separation and exit from mitosis. Despite its importance, the role of APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, the Arabidopsis thaliana APC/C subunit APC10 was characterized and shown to functionally complement an apc10 yeast mutant. The APC10 protein was located in specific nuclear bodies, most probably resulting from its association with the proteasome complex. An apc10 Arabidopsis knockout mutant strongly impaired female gametogenesis. Surprisingly, constitutive overexpression of APC10 enhanced leaf size. Through kinematic analysis, the increased leaf size was found to be due to enhanced rates of cell division during the early stages of leaf development and, at the molecular level, by increased APC/C activity as measured by an amplification of the proteolysis rate of the mitotic cyclin, CYCB1;1.

Short Title:The Plant Journal
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