Publication Type: | Journal Article |
Year of Publication: | 2012 |
Authors: | Pound, MP, French, AP, Wells, DM, Bennett, MJ, Pridmore, TP |
Journal: | The Plant Cell |
Volume: | 24 |
Issue: | 4 |
Date Published: | 2012 |
ISBN Number: | 10404651 |
Abstract: | It is increasingly important in life sciences that many cell-scale and tissue-scale measurements are quantified from confocal microscope images. However, extracting and analyzing large-scale confocal image data sets represents a major bottleneck for researchers. To aid this process, CellSeT software has been developed, which utilizes tissue-scale structure to help segment individual cells. We provide examples of how the CellSeT software can be used to quantify fluorescence of hormone-responsive nuclear reporters, determine membrane protein polarity, extract cell and tissue geometry for use in later modeling, and take many additional biologically relevant measures using an extensible plug-in toolset. Application of CellSeT promises to remove subjectivity from the resulting data sets and facilitate higher-throughput, quantitative approaches to plant cell research. |
URL: | http://www.jstor.org/stable/41549810 |
Short Title: | The Plant Cell |