IBIS-Flora

Angiosperm Flora of India

Comprehensive Sequence Analysis of 24,783 Barley Full-Length cDNAs Derived from 12 Clone Libraries

Publication Type:Journal Article
Year of Publication:2011
Authors:Matsumoto, T, Tanaka, T, Sakai, H, Amano, N, Kanamori, H, Kurita, K, Kikuta, A, Kamiya, K, Yamamoto, M, Ikawa, H, Fujii, N, Hori, K, Itoh, T, Sato, K
Journal:Plant Physiology
Volume:156
Issue:1
Date Published:2011
ISBN Number:00320889
Keywords:Hordeum, Hordeum vulgare, Oryza, Oryza sativa
Abstract:

Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 representative sequences were obtained: 17,773 were novel barley FLcDNAs, and 1,699 were barley specific. Highly conserved genes were found in the barley FLcDNA sequences for 721 of 881 rice (Oryza sativa) trait genes with 50% or greater identity. These FLcDNA resources from our Haruna Nijo cDNA libraries and the full-length sequences of representative clones will improve our understanding of the biological functions of genes in barley, which is the cereal crop with the fourth highest production in the world, and will provide a powerful tool for annotating the barley genome sequences that will become available in the near future.

URL:http://www.jstor.org/stable/41434580
Short Title:Plant Physiology
Fri, 2014-01-24 22:00 -- admin
https://secure.gravatar.com/avatar/5ade1b012674ce3dd941e2ea5dd15cc1.jpg?d=https%3A//flora.indianbiodiversity.org/sites/all/modules/patches/contrib/gravatar/avatar.png&s=100&r=G
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith