|Publication Type:||Journal Article|
|Year of Publication:||2012|
|Authors:||Mishra, RK, Gangadhar, BH, Nookaraju, A, Kumar, S, Park, SW|
|Keywords:||expressed sequence tags, Medicago, Medicago truncatula, microsatellites, Pisum sativum, polymorphism, simple sequence repeats|
With 1 figure and 3 tablesAbstract Simple sequence repeats (SSRs) derived from expressed sequence tags (ESTs) are important resources for gene discovery and mapping. In this study, we developed EST-based SSR (eSSRs) markers and assessed their ability in mapping and transferability. A total of 10 800 unigenes were detected from 18 522 pea EST sequences (December 2009). Screening of 10 800 unigenes by MISA (MIcroSAtellite) revealed 2612 (14.1%) eSSRs in 2395 (12.9%) SSR-containing ESTs from which 577 (24.1%) primer pairs were designed. The most abundant repeat motif identified in eSSR was mononucleotide (85.2%), followed by trinucleotide (10.6%) and dinucleotide (2.8%). Among 108 randomly selected primer pairs, 40 were assessed for mapping and 68 to test cross-species transferability in six leguminous species. Out of 40 primer pairs, 85% produced amplicons, 60% showed polymorphism and 47.5% were mapped. Furthermore, 68 primer pairs revealed high rate of transferability (48–85%) in leguminous species. High levels of polymorphism, reproducibility, presence of alleles (3.8/locus) and transferability revealed the potential use of these eSSR markers in molecular mapping, quantitative trait loci (QTL) analysis and comparative mapping in pea and other legumes.
|Short Title:||Plant Breeding|