IBIS-Flora

Angiosperm Flora of India

Development of sex-linked AFLP markers in Simmondsia chinensis

Publication Type:Journal Article
Year of Publication:2011
Authors:Agarwal, M, Shrivastava, N, Padh, H
Journal:Plant Breeding
Volume:130
Issue:1
Date Published:2011
ISBN Number:1439-0523
Keywords:AFLP, dioecious, molecular markers, sex-specific
Abstract:

With 2 figuresAbstract In most economically important dioecious plants such as Simmondsia chinensis (Jojoba), sex identification based on flowering can only be made after 1–5 years of plantation. Identification of sex-associated molecular markers for ascertaining sex at seedling stage is a desirable prerequisite for optimal plantation and economical benefits. Jojoba is being developed as a plantation crop in the arid regions of Western India due to the commercial benefits of the unique waxy content of its seeds. In the present study, sex-linked DNA markers have been identified in S. chinensis using the AFLP technique. From sixteen primers combinations used for AFLP analysis, two combinations EcoRI-TAC/MseI-GCG and EcoRI-GC/MseI-GCG resulted in amplification of sex-linked markers. Two male-specific markers of ∼525 bp and ∼325 bp were identified using the primer combinations EcoRI-GC/MseI-GCG and EcoRI-TAC/MseI-GCG, respectively. A female-specific marker of ∼270 bp was also identified with the primer combination EcoRI-TAC/MseI-GCG. In future, SCAR markers developed from these sex-specific markers will provide an effective and reliable method for sex identification.

URL:http://dx.doi.org/10.1111/j.1439-0523.2009.01749.x
Short Title:Plant Breeding
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