Publication Type: | Journal Article |
Year of Publication: | 2011 |
Authors: | Agarwal, M, Shrivastava, N, Padh, H |
Journal: | Plant Breeding |
Volume: | 130 |
Issue: | 1 |
Date Published: | 2011 |
ISBN Number: | 1439-0523 |
Keywords: | AFLP, dioecious, molecular markers, sex-specific |
Abstract: | With 2 figuresAbstract In most economically important dioecious plants such as Simmondsia chinensis (Jojoba), sex identification based on flowering can only be made after 1–5 years of plantation. Identification of sex-associated molecular markers for ascertaining sex at seedling stage is a desirable prerequisite for optimal plantation and economical benefits. Jojoba is being developed as a plantation crop in the arid regions of Western India due to the commercial benefits of the unique waxy content of its seeds. In the present study, sex-linked DNA markers have been identified in S. chinensis using the AFLP technique. From sixteen primers combinations used for AFLP analysis, two combinations EcoRI-TAC/MseI-GCG and EcoRI-GC/MseI-GCG resulted in amplification of sex-linked markers. Two male-specific markers of ∼525 bp and ∼325 bp were identified using the primer combinations EcoRI-GC/MseI-GCG and EcoRI-TAC/MseI-GCG, respectively. A female-specific marker of ∼270 bp was also identified with the primer combination EcoRI-TAC/MseI-GCG. In future, SCAR markers developed from these sex-specific markers will provide an effective and reliable method for sex identification. |
URL: | http://dx.doi.org/10.1111/j.1439-0523.2009.01749.x |
Short Title: | Plant Breeding |