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Extracellular Nucleotides Elicit Cytosolic Free Calcium Oscillations in Arabidopsis

Publication Type:Journal Article
Year of Publication:2010
Authors:Tanaka, K, Swanson, SJ, Gilroy, S, Stacey, G
Journal:Plant Physiology
Volume:154
Issue:2
Date Published:2010
ISBN Number:00320889
Abstract:

Extracellular ATP induces a rise in the level of cytosolic free calcium ([Ca²⁺] cyt ) in plant cells. To expand our knowledge about the function of extracellular nucleotides in plants, the effects of several nucleotide analogs and pharmacological agents on [Ca²⁺] cyt changes were studied using transgenic Arabidopsis (Arabidopsis thaliana) expressing aequorin or the fluorescence resonance energy transfer-based Ca²⁺ sensor Yellow Cameleon 3.6. Exogenously applied CTP caused elevations in [Ca²⁺] cyt that displayed distinct time- and dose-dependent kinetics compared with the purine nucleotides ATP and GTP. The inhibitory effects of antagonists of mammalian P2 receptors and calcium influx inhibitors on nucleotide-induced [Ca²⁺] cyt elevations were distinct between CTP and purine nucleotides. These results suggest that distinct recognition systems may exist for the respective types of nucleotides. Interestingly, a mutant lacking the heterotrimeric G protein Gβ-subunit exhibited a remarkably higher [Ca²⁺] cyt elevation in response to all tested nucleotides in comparison with the wild type. These data suggest a role for Gβ in negatively regulating extracellular nucleotide signaling and point to an important role for heterotrimeric G proteins in modulating the cellular effects of extracellular nucleotides. The addition of extracellular nucleotides induced multiple temporal [Ca²⁺] cyt oscillations, which could be localized to specific root cells. The oscillations were attenuated by a vesicle-trafficking inhibitor, indicating that the oscillations likely require ATP release via exocytotic secretion. The results reveal new molecular details concerning extracellular nucleotide signaling in plants and the importance of fine control of extracellular nucleotide levels to mediate specific plant cell responses.

URL:http://www.jstor.org/stable/20779826
Short Title:Plant Physiology
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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith