|Publication Type:||Journal Article|
|Year of Publication:||2010|
|Authors:||Boutant, E, Didier, P, Niehl, A, Mely, Y, Ritzenthaler, C, Heinlein, M|
|Journal:||The Plant Journal|
|Keywords:||GFP, in vivo protein interactions, movement protein, tobacco mosaic virus|
We describe a simple fluorescent protein-based method to investigate interactions with a viral movement protein in living cells that relies on the in vivo re-localization of proteins in the presence of their interaction partners. We apply this method in combination with fluorescence lifetime imaging microscopy (FLIM) to demonstrate that a domain of the Tobacco mosaic virus (TMV) movement protein (MP) previously predicted to mediate protein:protein interactions is dispensable for these contacts. We suggest that this method can be generalized for analysis of other protein interactions in planta.
|Short Title:||The Plant Journal|