Angiosperm Flora of India

Fluorescent protein recruitment assay for demonstration and analysis of in vivo protein interactions in plant cells and its application to Tobacco mosaic virus movement protein

Publication Type:Journal Article
Year of Publication:2010
Authors:Boutant, E, Didier, P, Niehl, A, Mely, Y, Ritzenthaler, C, Heinlein, M
Journal:The Plant Journal
Date Published:2010
ISBN Number:1365-313X
Keywords:GFP, in vivo protein interactions, movement protein, tobacco mosaic virus

We describe a simple fluorescent protein-based method to investigate interactions with a viral movement protein in living cells that relies on the in vivo re-localization of proteins in the presence of their interaction partners. We apply this method in combination with fluorescence lifetime imaging microscopy (FLIM) to demonstrate that a domain of the Tobacco mosaic virus (TMV) movement protein (MP) previously predicted to mediate protein:protein interactions is dispensable for these contacts. We suggest that this method can be generalized for analysis of other protein interactions in planta.

Short Title:The Plant Journal
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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith