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Fluorescent protein recruitment assay for demonstration and analysis of in vivo protein interactions in plant cells and its application to Tobacco mosaic virus movement protein

Publication Type:Journal Article
Year of Publication:2010
Authors:Boutant, E, Didier, P, Niehl, A, Mely, Y, Ritzenthaler, C, Heinlein, M
Journal:The Plant Journal
Volume:62
Issue:1
Date Published:2010
ISBN Number:1365-313X
Keywords:GFP, in vivo protein interactions, movement protein, tobacco mosaic virus
Abstract:

We describe a simple fluorescent protein-based method to investigate interactions with a viral movement protein in living cells that relies on the in vivo re-localization of proteins in the presence of their interaction partners. We apply this method in combination with fluorescence lifetime imaging microscopy (FLIM) to demonstrate that a domain of the Tobacco mosaic virus (TMV) movement protein (MP) previously predicted to mediate protein:protein interactions is dispensable for these contacts. We suggest that this method can be generalized for analysis of other protein interactions in planta.

URL:http://dx.doi.org/10.1111/j.1365-313X.2010.04126.x
Short Title:The Plant Journal
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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith