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Angiosperm Flora of India

Function and Evolution of a MicroRNA That Regulates a Ca²⁺-ATPase and Triggers the Formation of Phased Small Interfering RNAs in Tomato Reproductive Growth

Publication Type:Journal Article
Year of Publication:2011
Authors:Wang, Y, Itaya, A, Zhong, X, Wu, Y, Zhang, J, Van Der Knaap, E, Olmstead, R, Qi, Y, Ding, B
Journal:The Plant Cell
Volume:23
Issue:9
Date Published:2011
ISBN Number:10404651
Keywords:Lycopersicum, Solanaceae, Solanum
Abstract:

MicroRNAs (miRNAs) regulate a wide variety of biological processes in most eukaryotes. We investigated the function and evolution of miR4376 in the family Solanaceae. We report that the 22-nucleotide miR4376 regulates the expression of an autoinhibited Ca²⁺-ATPase, tomato (Solanum lycopersicum) ACA10, which plays a critical role in tomato reproductive growth. Deep phylogenetic mapping suggested (1) an evolution course of MIR4376 loci and posttranscriptional processing of premiR4376 as a likely limiting step for the evolution of miR4376, (2) an independent phylogenetic origin of the miR4376 target site in ACA10 homologs, and (3) alternative splicing as a possible mechanism of eliminating such a target in some ACA10 homologs. Furthermore, miR4376 triggers the formation of phased small interfering RNAs (siRNAs) from SI ACA10 and its Solanum tuberosum homolog. Together, our data provide experimental evidence of mi RNA-regulated expression of universally important Ca²⁺-ATPases. The miR4376-regulated expression of ACA10 itself, and possibly also the associated formation of phased siRNAs, may function as a novel layer of molecular mechanisms underlying tomato reproductive growth. Finally, our data suggest that the stochastic emergence of a miRNA-target gene combination involves multiple molecular events at the genomic, transcriptional, and posttranscriptional levels that may vary drastically in even closely related species.

URL:http://www.jstor.org/stable/41434691
Short Title:The Plant Cell
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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith