Angiosperm Flora of India

Functional identification of the phosphorylation sites of Arabidopsis PIN-FORMED3 for its subcellular localization and biological role

Publication Type:Journal Article
Year of Publication:2012
Authors:Ganguly, A, Lee, S-H, Cho, H-T
Journal:The Plant Journal
Date Published:2012
ISBN Number:1365-313X
Keywords:Arabidopsis thaliana, auxin, auxin transport, membrane polarity, phosphorylation, PIN-FORMED (PIN), root hair, vesicle trafficking

Directional cell-to-cell movement of auxin is mediated by asymmetrically localized PIN-FORMED (PIN) auxin efflux transporters. The polar localization of PINs has been reported to be modulated by phosphorylation. In this study, the function of the phosphorylation sites of the PIN3 central hydrophilic loop (HL) was characterized. The phosphorylation sites were located in two conserved neighboring motifs, RKSNASRRSF(/L) and TPRPSNL, where the former played a more decisive role than the latter. Mutations of these phosphorylatable residues disrupted in planta phosphorylation of PIN3 and its subcellular trafficking, and caused defects in PIN3-mediated biological processes such as auxin efflux activity, auxin maxima formation, root growth, and root gravitropism. Because the defective intracellular trafficking behaviors of phospho-mutated PIN3 varied according to cell type, phosphorylation codes in PIN3-HL are likely to operate in a cell-type-specific manner.

Short Title:The Plant Journal
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