Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses

Publication Type:Journal Article
Year of Publication:2012
Authors:Brandt, R, Salla-Martret, M, Bou-Torrent, J, Musielak, T, Stahl, M, Lanz, C, Ott, F, Schmid, M, Greb, T, Schwarz, M, Choi, S-B, M. Barton, K, Reinhart, BJ, Liu, T, Quint, M, Palauqui, J-C, Martínez-Garcia, JF, Wenkel, S
Journal:The Plant Journal
Volume:72
Issue:1
Date Published:2012
ISBN Number:1365-313X
Keywords:Arabidopsis thaliana, auxin, HD-ZIPII, HD-ZIPIII, leaf development, shade avoidance
Abstract:

Unlike the situation in animals, the final morphology of the plant body is highly modulated by the environment. During Arabidopsis development, intrinsic factors provide the framework for basic patterning processes. CLASS III HOMEODOMAIN LEUCINE ZIPPER (HD-ZIPIII) transcription factors are involved in embryo, shoot and root patterning. During vegetative growth HD-ZIPIII proteins control several polarity set-up processes such as in leaves and the vascular system. We have identified several direct target genes of the HD-ZIPIII transcription factor REVOLUTA (REV) using a chromatin immunoprecipitation/DNA sequencing (ChIP-Seq) approach. This analysis revealed that REV acts upstream of auxin biosynthesis and affects directly the expression of several class II HD-ZIP transcription factors that have been shown to act in the shade-avoidance response pathway. We show that, as well as involvement in basic patterning, HD-ZIPIII transcription factors have a critical role in the control of the elongation growth that is induced when plants experience shade. Leaf polarity is established by the opposed actions of HD-ZIPIII and KANADI transcription factors. Finally, our study reveals that the module that consists of HD-ZIPIII/KANADI transcription factors controls shade growth antagonistically and that this antagonism is manifested in the opposed regulation of shared target genes.

URL:http://dx.doi.org/10.1111/j.1365-313X.2012.05049.x
Short Title:The Plant Journal
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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith