|Publication Type:||Journal Article|
|Year of Publication:||2011|
|Authors:||Gupta, iv, S, Li, C, Loughman, R, Cakir, M, Westcott, S, Lance, R|
|Keywords:||double haploid, Hordeum, Hordeum vulgare, Mapping, simple sequence repeat|
With 2 figures and 4 tablesAbstract Net type net blotch (NTNB) is an important barley disease in Australia and elsewhere, inducing significant yield reduction. ‘WPG8412’ was resistant against Australian NTNB isolates 97NB1 and NB73, whereas ‘Pompadour’ and ‘Stirling’ showed differential responses to these isolates. Using these lines, three F1-derived double haploid populations that comprised 194 double haploid lines (DHLs) of ‘WPG8412’ ×‘Stirling’, 116 DHLs of ‘WPG8412’ × ‘Pompadour’ and 206 DHLs of ‘Pompadour’ × ‘Stirling’ were tested against these two isolates. Bimodal segregation indicated a major gene for resistance was operative: in ‘WPG8412’ × ‘Stirling’ against 97NB1; in ‘WPG8412’×‘Pompadour’ against NB73; and in ‘Pompadour’ × ‘Stirling’ against either of the isolate. This major gene was mapped using simple sequence repeat (SSR) markers on chromosome 6H in the centromeric region in all three populations. This work demonstrated that 6H region controlling NTNB is a complex locus, where at least three alleles or closely linked genes are possible in these parents against these isolates. This is a first report of 6H complexity for NTNB resistance against Australian isolates.
|Short Title:||Plant Breeding|