|Publication Type:||Journal Article|
|Year of Publication:||2012|
|Authors:||Masood, S, WIMMER, MA, Witzel, K, Zörb, C, MÜHLING, KH|
|Journal:||Journal of Agronomy and Crop Science|
|Keywords:||apoplast, B toxicity, multiple stress, Salinity, symplast, wheat|
Salinity and boron (B) toxicity often occur simultaneously and may have interactive effects on plant responses. This study aims at further investigating long-term effects of salinity, B toxicity and their combination on wheat plants. Plants grown in hydroponics were treated with 2.5 μm H3BO3 (control), 75 mm NaCl, 200 μm H3BO3 or 75 mm NaCl + 200 μm H3BO3 and were analysed on a subcellular level 6 weeks after germination. Shoot fresh and dry weight (DW), water content, transpiration rate and osmolality were reduced, while Na+ and Cl− concentrations increased under salinity stress. However, Cl− was reduced in all compartments by an additional application of B toxicity. At adequate B supply, NaCl increased apoplastic and symplastic soluble B concentrations, whereas the total B content remained unchanged. At high B level, however, soluble and total B was reduced by additional salt stress. Despite this antagonistic effect of a combined salt and B toxicity stress on Cl− and B concentrations, an additive effect was observed regarding shoot fresh weight reduction. Our results confirm an alleviating effect of the combined stresses on toxic ion concentrations, which did not prevent additive growth reductions.
|Short Title:||Journal of Agronomy and Crop Science|