Isolation and characterisation of the first microsatellite markers for Cyperus rotundus

Publication Type:Journal Article
Year of Publication:2011
Authors:ARIAS, RS, MOLIN, WT, RAY, JD, PEEL, MD, SCHEFFLER, BE
Journal:Weed Research
Volume:51
Issue:5
Date Published:2011
ISBN Number:1365-3180
Keywords:Cyperus, Cyperus esculentus, Cyperus rotundus, minisatellite, molecular markers, nutgrass, purple nutsedge, simple sequence repeat, simple sequence repeats, STR, yellow nutsedge
Abstract:

Arias RS, Molin WT, Ray JD, Peel MD & Scheffler BE (2011). Isolation and characterisation of the first microsatellite markers for Cyperus rotundus. Weed Research51, 451–460.Summary This is the first report of microsatellite markers for Cyperus rotundus. A total of 191 sequence-specific microsatellite markers were isolated and used to screen 12 accessions of C. rotundus and one accession of Cyperus esculentus collected from 10 different countries. Polymorphisms were observed in 49% of the markers tested, 22% of the markers were monomorphic and 29% had weak or no amplification. The best 57 markers are reported, and cluster analysis was used to analyse their resolving power. BLASTx screening of the contig sequences was also performed. Multiallelic loci over all samples ranged from 24% to 60%. The maximum number of alleles detected by the markers suggests a polyploidy nature of all C. rotundus accessions tested, except for the sample N25-Brazil. Chromosome number was determined for N12-Taiwan and used as an internal flow cytometry standard to estimate the amount of DNA within haploid nuclei of the remaining material. Chromosome numbers estimated for C. rotundus were 16 and 24. The markers identified in this study can be used for the identification of biotypes and detection of potential crosses of C. rotundus, to implement management practices for the effective control of this weed.

URL:http://dx.doi.org/10.1111/j.1365-3180.2011.00861.x
Short Title:Weed Research
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