IBIS-Flora

Angiosperm Flora of India

Live-Cell Imaging Reveals Periarbuscular Membrane Domains and Organelle Location in Medicago truncatula Roots during Arbuscular Mycorrhizal Symbiosis

Publication Type:Journal Article
Year of Publication:2009
Authors:Pumplin, N, Harrison, MJ
Journal:Plant Physiology
Volume:151
Issue:2
Date Published:2009
ISBN Number:00320889
Keywords:Medicago
Abstract:

In the arbuscular mycorrhizal symbiosis, the fungal symbiont colonizes root cortical cells, where it establishes differentiated hyphae called arbuscules. As each arbuscule develops, the cortical cell undergoes a transient reorganization and envelops the arbuscule in a novel symbiosis-specific membrane, called the periarbuscular membrane. The periarbuscular membrane, which is continuous with the plant plasma membrane of the cortical cell, is a key interface in the symbiosis; however, relatively little is known of its composition or the mechanisms of its development. Here, we used fluorescent protein fusions to obtain both spatial and temporal information about the protein composition of the periarbuscular membrane. The data indicate that the periarbuscular membrane is composed of at least two distinct domains, an "arbuscule branch domain" that contains the symbiosis-specific phosphate transporter, MtPT4, and an "arbuscule trunk domain" that contains MtBcpl. This suggests a developmental transition from plasma membrane to periarbuscular membrane, with biogenesis of a novel membrane domain associated with the repeated dichotomous branching of the hyphae. Additionally, we took advantage of available organellespecific fluorescent marker proteins to further evaluate cells during arbuscule development and degeneration. The threedimensional data provide new insights into relocation of Golgi and peroxisomes and also illustrate that cells with arbuscules can retain a large continuous vacuolar system throughout development.

URL:http://www.jstor.org/stable/40537813
Short Title:Plant Physiology
Taxonomic name: 
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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith