Publication Type: | Journal Article |
Year of Publication: | 2012 |
Authors: | Hossain, MShakhawat, Liao, J, James, EK, Sato, S, Tabata, S, Jurkiewicz, A, Madsen, LH, Stougaard, J, Ross, L, Szczyglowski, K |
Journal: | Plant Physiology |
Volume: | 160 |
Issue: | 2 |
Date Published: | 2012 |
ISBN Number: | 00320889 |
Keywords: | Lotus |
Abstract: | Remodeling of the plant cell cytoskeleton precedes symbiotic entry of nitrogen-fixing bacteria within the host plant roots. Here we identify a Lotus japonicus gene encoding a predicted ACTIN-RELATED PROTEIN COMPONENT1 (ARPC1) as essential for rhizobial infection but not for arbuscular mycorrhiza symbiosis. In other organisms ARPC1 constitutes a subunit of the ARP2/3 complex, the major nucleator of Y-branched actin filaments. The L. japonicus arpc1 mutant showed a distorted trichome phenotype and was defective in epidermal infection thread formation, producing mostly empty nodules. A few partially colonized nodules that did form in arpc1 contained abnormal infections. Together with previously described L. japonicus Nckassociated protein1 and 121F-specific p53 inducible RNA mutants, which are also impaired in the accommodation of rhizobia, our data indicate that ARPC1 and, by inference a suppressor of cAMP receptor/WASP-family verpolin homologous protein-ARP2/3 pathway, must have been coopted during evolution of nitrogen-fixing symbiosis to specifically mediate bacterial entry. |
URL: | http://www.jstor.org/stable/41694810 |
Short Title: | Plant Physiology |
Lotus japonicus ARPC1 Is Required for Rhizobial Infection
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