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A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO₂ in Chlamydomonas reinhardtii

Publication Type:Journal Article
Year of Publication:2010
Authors:Renberg, L, Johansson, AI, Shutova, T, Stenlund, H, Aksmann, A, RAVEN, JOHNA, Gardeström, P, Moritz, T, SAMUELSSON, GÖRAN
Journal:Plant Physiology
Volume:154
Issue:1
Date Published:2010
ISBN Number:00320889
Abstract:

Using a gas chromatography-mass spectrometry-time of flight technique, we determined major metabolite changes during induction of the carbon-concentrating mechanism in the unicellular green alga Chlamydomonas reinhardtii. In total, 128 metabolites with significant differences between high- and low-CO₂-grown cells were detected, of which 82 were wholly or partially identified, including amino acids, lipids, and carbohydrates. In a 24-h time course experiment, we show that the amino acids serine and phenylalanine increase transiently while aspartate and glutamate decrease after transfer to low CO₂. The biggest differences were typically observed 3 h after transfer to low-CO₂ conditions. Therefore, we made a careful metabolomic examination at the 3-h time point, comparing low-CO₂ treatment to high-CO₂ control. Five metabolites involved in photorespiration, 11 amino acids, and one lipid were increased, while six amino acids and, interestingly, 21 lipids were significantly lower. Our conclusion is that the metabolic pattern during early induction of the carbon-concentrating mechanism fit a model where photorespiration is increasing.

URL:http://www.jstor.org/stable/20779757
Short Title:Plant Physiology
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