MORPHEUS' MOLECULE1 Is Required to Prevent Aberrant RNA Transcriptional Read-Through in Arabidopsis

Publication Type:Journal Article
Year of Publication:2010
Authors:Zhou, Y, Zhang, J, Lin, H, Guo, G, Guo, Y
Journal:Plant Physiology
Date Published:2010
ISBN Number:00320889

Several pathways function to remove aberrant mRNA in eukaryotic cells; however, the exact mechanisms underlying the restriction of aberrant mRNA transcription are poorly understood. In this study, we found that MORPHEUS' MOLECULE1 (MOM1) is a key component of this regulatory machinery. The Arabidopsis (Arabidopsis thaliana) mom1-44 mutation was identified by luciferase imaging in transgenic plants harboring a cauliflower mosaic virus 35S promoter-LUCIFERASE transgene lacking the 3′-untranslated region. In the mom1-44 mutant, transcriptional read-though occurred in genes with an aberrant RNA structure. Analysis of an RNA-dependent RNA polymerase2 mom1 double mutant revealed that the RNA-directed DNA methylation pathway is not involved in this regulatory process. Moreover, the prevention of aberrant mRNA transcriptional read-through by MOM1 is gene locus and transgene copy number independent.

Short Title:Plant Physiology
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