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Network Analysis of Enzyme Activities and Metabolite Levels and Their Relationship to Biomass in a Large Panel of Arabidopsis Accessions

Publication Type:Journal Article
Year of Publication:2010
Authors:Sulpice, R, Trenkamp, S, Steinfath, M, Usadel, B, Gibon, Y, Witucka-Wall, H, PYL, EVA-THERESA, Tschoep, H, Steinhauser, MCaroline, Guenther, M, Hoehne, M, Rohwer, JM, Altmann, T, Fernie, AR, Stitt, M
Journal:The Plant Cell
Volume:22
Issue:8
Date Published:2010
ISBN Number:10404651
Abstract:

Natural genetic diversity provides a powerful resource to investigate how networks respond to multiple simultaneous changes. In this work, we profile maximum catalytic activities of 37 enzymes from central metabolism and generate a matrix to investigate species-wide connectivity between metabolites, enzymes, and biomass. Most enzyme activities change in a highly coordinated manner, especially those in the Calvin-Benson cycle. Metabolites show coordinated changes in defined sectors of metabolism. Little connectivity was observed between maximum enzyme activities and metabolites, even after applying multivariate analysis methods. Measurements of posttranscriptional regulation will be required to relate these two functional levels. Individual enzyme activities correlate only weakly with biomass. However, when they are used to estimate protein abundances, and the latter are summed and expressed as a fraction of total protein, a significant positive correlation to biomass is observed. The correlation is additive to that obtained between starch and biomass. Thus, biomass is predicted by two independent integrative metabolic biomarkers: preferential investment in photosynthetic machinery and optimization of carbon use.

URL:http://www.jstor.org/stable/20780609
Short Title:The Plant Cell
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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith