IBIS-Flora

Angiosperm Flora of India

A new phytoplasma associated with witches’-broom on Japanese maple in China

Publication Type:Journal Article
Year of Publication:2012
Authors:Li, Z-N, ZHANG, L, Zhao, L, Wu, Y-F
Journal:Forest Pathology
Volume:42
Issue:5
Date Published:2012
ISBN Number:1439-0329
Keywords:Acer
Abstract:

In September 2011, five Japanese maple (Acer palmatum Thunb.) trees with symptoms of witches’-broom were observed growing near each other at a maple grove in Northwest A&F University, Yangling, Shaanxi Province, China. Pleomorphic phytoplasma-like bodies were observed in the phloem sieve tube elements of symptomatic plants under transmission electron microscope (TEM). The presence of phytoplasma was further confirmed by a nested polymerase chain reaction (PCR), which amplified a 1.2-kb fragment using universal primer pair R16mF2/R16mR1 followed by further amplification using primer pair R16F2n/R16R2. Phylogenetic analysis and gel-based restriction fragment length polymorphism (RFLP) analysis demonstrated that the Japanese maple witches’-broom was associated with phytoplasma belonging to subgroup 16SrI-D. This is the first report of a phytoplasma disease of Japanese maple.

URL:http://dx.doi.org/10.1111/j.1439-0329.2012.00769.x
Short Title:Forest Pathology
Taxonomic name: 
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