Publication Type: | Journal Article |
Year of Publication: | 2012 |
Authors: | Li, Z-N, ZHANG, L, Zhao, L, Wu, Y-F |
Journal: | Forest Pathology |
Volume: | 42 |
Issue: | 5 |
Date Published: | 2012 |
ISBN Number: | 1439-0329 |
Keywords: | Acer |
Abstract: | In September 2011, five Japanese maple (Acer palmatum Thunb.) trees with symptoms of witches’-broom were observed growing near each other at a maple grove in Northwest A&F University, Yangling, Shaanxi Province, China. Pleomorphic phytoplasma-like bodies were observed in the phloem sieve tube elements of symptomatic plants under transmission electron microscope (TEM). The presence of phytoplasma was further confirmed by a nested polymerase chain reaction (PCR), which amplified a 1.2-kb fragment using universal primer pair R16mF2/R16mR1 followed by further amplification using primer pair R16F2n/R16R2. Phylogenetic analysis and gel-based restriction fragment length polymorphism (RFLP) analysis demonstrated that the Japanese maple witches’-broom was associated with phytoplasma belonging to subgroup 16SrI-D. This is the first report of a phytoplasma disease of Japanese maple. |
URL: | http://dx.doi.org/10.1111/j.1439-0329.2012.00769.x |
Short Title: | Forest Pathology |
A new phytoplasma associated with witches’-broom on Japanese maple in China
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