Publication Type: | Journal Article |
Year of Publication: | 2012 |
Authors: | RASUL, S, DUBREUIL-MAURIZI, C, LAMOTTE, O, KOEN, E, POINSSOT, B, ALCARAZ, G, WENDEHENNE, D, JEANDROZ, S |
Journal: | Plant, Cell & Environment |
Volume: | 35 |
Issue: | 8 |
Date Published: | 2012 |
ISBN Number: | 1365-3040 |
Keywords: | Calcium, nitrate reductase, Plant defence, reactive oxygen species. |
Abstract: | Nitric oxide (NO) regulates a wide range of plant processes from development to environmental adaptation. In this study, we investigated the production and/or function of NO in Arabidopsis thaliana leaf discs and plants elicited by oligogalacturonides (OGs) and challenged with Botrytis cinerea. We provided evidence that OGs triggered a fast and long lasting NO production which was Ca2+ dependent and involved nitrate reductase (NR). Accordingly, OGs triggered an increase of both NR activity and transcript accumulation. NO production was also sensitive to the mammalian NO synthase inhibitor L-NAME. Intriguingly, we showed that L-NAME affected NO production by interfering with NR activity, thus questioning the mechanisms of how this compound impairs NO synthesis in plants. We further demonstrated that NO modulates RBOHD-mediated reactive oxygen species (ROS) production and participates in the regulation of OG-responsive genes such as anionic peroxidase (PER4) and a β-1,3-glucanase. Mutant plants impaired in PER4 and β-1,3-glucanase, as well as Col-0 plants treated with the NO scavenger cPTIO, were more susceptible to B. cinerea. Taken together, our investigation deciphers part of the mechanisms linking NO production, NO-induced effects and basal resistance to B. cinerea. |
URL: | http://dx.doi.org/10.1111/j.1365-3040.2012.02505.x |
Short Title: | Plant, Cell & Environment |