PETAL LOSS is a boundary gene that inhibits growth between developing sepals in Arabidopsis thaliana

Publication Type:Journal Article
Year of Publication:2012
Authors:Lampugnani, ER, Kilinc, A, Smyth, DR
Journal:The Plant Journal
Volume:71
Issue:5
Date Published:2012
ISBN Number:1365-313X
Keywords:Arabidopsis, boundary gene, cotyledon, CUP-SHAPED COTYLEDON, perianth, PETAL LOSS, trihelix
Abstract:

Flower primordia are partitioned by boundaries during their early development. Such boundaries occur between whorls of organs, and also between organs within whorls. PETAL LOSS (PTL) is a trihelix transcription factor gene that is expressed in boundaries between sepal primordia in the outer whorl. Over-expression of PTL results in growth suppression suggesting that PTL normally inhibits growth between newly arising sepals. We have tested this by examining the consequences of loss of PTL function using confocal imaging. The size of the inter-sepal zone in stage 4 buds expands radially by 35–40% in ptl-1 mutants as a consequence of additional cell proliferation. There is no change in the size of PTL-expressing cells. PTL expression does not overlap with the sites of petal initiation identified using the DR5 auxin response reporter. The latter are closer to the centre of the flower. Thus the consequence of loss of PTL function on petal initiation is indirect, perhaps through interference with a mobile petal-initiation signal or movement of the PTL protein. CUP-SHAPED COTYLEDON (CUC) genes are also involved in defining inter-sepal boundaries. However, genetic studies combining ptl with loss of cuc1 function, and gain of CUC function in extra early petals-1 (miR164c) mutants, have revealed that CUC and PTL act differently. CUC suppresses growth of sepal tissues from the boundary region whereas PTL acts to keep the size of the boundary in check.

URL:http://dx.doi.org/10.1111/j.1365-313X.2012.05023.x
Short Title:The Plant Journal
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