Publication Type: | Journal Article |
Year of Publication: | 2012 |
Authors: | Hu, J, Wang, K, Huang, W, Liu, G, Gao, Y, Wang, J, Huang, Q, Ji, Y, Qin, X, Wan, L, Zhu, R, Li, S, Yang, D, Zhu, Y |
Journal: | The Plant Cell |
Volume: | 24 |
Issue: | 1 |
Date Published: | 2012 |
ISBN Number: | 10404651 |
Keywords: | glycine |
Abstract: | The cytoplasmic male sterility (CMS) phenotype in plants can be reversed by the action of nuclear-encoded fertility restorer (Rf) genes. The molecular mechanism involved in Rf gene-mediated processing of CMS-associated transcripts is unclear, as are the identities of other proteins that may be involved in the CbAS-Rf interaction. In this study, we cloned the restorer gene Rf5 for Hong-Lian CMS in rice and studied its fertility restoration mechanism with respect to the processing of the CMS-associated transcript atp6-orfH79. RF5, a pentatricopeptide repeat (PPR) protein, was unable to bind to this CMSassociated transcript; however, a partner protein of RF5 (GRP162, a Gly-rich protein encoding 162 amino acids) was identified to bind to atp6-orfH79. GRP162 was found to physically interact with RF5 and to bind to atp6-orfH79 via an RNA recognition motif. Furthermore, we found that RF5 and GRP162 are both components of a restoration of fertility complex (RFC) that is 400 to 500 kD in size and can cleave CMS-associated transcripts in vitro. Evidence that a PPR protein interacts directly with a Gly-rich protein to form a subunit of the RFC provides a new perspective on the molecular mechanisms underlying fertility restoration. |
URL: | http://www.jstor.org/stable/41433953 |
Short Title: | The Plant Cell |
The Rice Pentatricopeptide Repeat Protein RF5 Restores Fertility in Hong-Lian Cytoplasmic Male-Sterile Lines via a Complex with the Glycine-Rich Protein GRP162
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