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Angiosperm Flora of India

Unlocking the Barley Genome by Chromosomal and Comparative Genomics

Publication Type:Journal Article
Year of Publication:2011
Authors:Mayer, KFX, Martis, M, HEDLEY, PETEE, Šimková, H, LIU, HUI, MORRIS, JENNYA, Steuernagel, B, Taudien, S, Roessner, S, Gundlach, H, Kubaláková, M, Suchánková, P, Murat, F, Felder, M, Nussbaumer, T, Graner, A, Salse, J, Endo, T, Sakai, H, Tanaka, T, ltoh, T, Sato, K, Platzer, M, Matsumoto, T, Scholz, U, Doležel, J, Waugh, R, Stein, N
Journal:The Plant Cell
Volume:23
Issue:4
Date Published:2011
ISBN Number:10404651
Keywords:Brachypodium, Hordeum, Hordeum vulgare, Oryza, Oryza sativa, Triticum
Abstract:

We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign ~86% of the estimated ~32,000 barley [Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum [Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.

URL:http://www.jstor.org/stable/41433389
Short Title:The Plant Cell
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