IBIS-Flora

Angiosperm Flora of India

Variation in potential effector genes distinguishing Australian and non-Australian isolates of the cotton wilt pathogen Fusarium oxysporum f.sp. vasinfectum

Publication Type:Journal Article
Year of Publication:2011
Authors:Chakrabarti, A, Rep, M, WANG, B, Ashton, A, Dodds, P, Ellis, J
Journal:Plant Pathology
Volume:60
Issue:2
Date Published:2011
ISBN Number:1365-3059
Keywords:cotton wilt, effector genes and proteins, Fusarium oxysporum formae speciales, Gossypium, Gossypium spp.
Abstract:

This study identified genes that distinguish Australian Fusarium oxysporum f.sp. vasinfectum (Fov) isolates from related co-localized non-pathogenic F. oxysporum isolates and from non-Australian Fov isolates. One gene is a homologue of the F. oxysporum f.sp. lycopersici (Fol) effector gene SIX6, encoding a 215-residue cysteine-rich secreted protein. The Six6 proteins from Fol and Fov contained eight conserved cysteine residues, five of which occurred in the highly diverged 48-amino-acid region where FovSix6 differs from FolSix6 at 32 residues. Two other potential effector genes, PEP1 and PEP2, were identified in a cDNA library of Fov genes expressed during infection of cotton. The presence of FovSIX6 and other differences in DNA fingerprints clearly distinguished Australian Fov isolates from non-Australian Fov isolates and these differences further support the hypothesis based on earlier phylogenetic analysis that Australian Fov is different from Fov in other cotton-growing areas. A specific diagnostic for Fov based on FovSIX6 is described.

URL:http://dx.doi.org/10.1111/j.1365-3059.2010.02363.x
Short Title:Plant Pathology
Taxonomic name: 
Fri, 2014-01-24 22:18 -- admin
https://secure.gravatar.com/avatar/5ade1b012674ce3dd941e2ea5dd15cc1.jpg?d=https%3A//flora.indianbiodiversity.org/sites/all/modules/patches/contrib/gravatar/avatar.png&s=100&r=G
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith