Publication Type: | Journal Article |
Year of Publication: | 2011 |
Authors: | Chakrabarti, A, Rep, M, WANG, B, Ashton, A, Dodds, P, Ellis, J |
Journal: | Plant Pathology |
Volume: | 60 |
Issue: | 2 |
Date Published: | 2011 |
ISBN Number: | 1365-3059 |
Keywords: | cotton wilt, effector genes and proteins, Fusarium oxysporum formae speciales, Gossypium, Gossypium spp. |
Abstract: | This study identified genes that distinguish Australian Fusarium oxysporum f.sp. vasinfectum (Fov) isolates from related co-localized non-pathogenic F. oxysporum isolates and from non-Australian Fov isolates. One gene is a homologue of the F. oxysporum f.sp. lycopersici (Fol) effector gene SIX6, encoding a 215-residue cysteine-rich secreted protein. The Six6 proteins from Fol and Fov contained eight conserved cysteine residues, five of which occurred in the highly diverged 48-amino-acid region where FovSix6 differs from FolSix6 at 32 residues. Two other potential effector genes, PEP1 and PEP2, were identified in a cDNA library of Fov genes expressed during infection of cotton. The presence of FovSIX6 and other differences in DNA fingerprints clearly distinguished Australian Fov isolates from non-Australian Fov isolates and these differences further support the hypothesis based on earlier phylogenetic analysis that Australian Fov is different from Fov in other cotton-growing areas. A specific diagnostic for Fov based on FovSIX6 is described. |
URL: | http://dx.doi.org/10.1111/j.1365-3059.2010.02363.x |
Short Title: | Plant Pathology |
Variation in potential effector genes distinguishing Australian and non-Australian isolates of the cotton wilt pathogen Fusarium oxysporum f.sp. vasinfectum
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